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Construct a volcano plot to visualise the changes in fold change and p values from health to sepsis. (By excel) If you assume p<0.05 is significant and prioritise two-fold increased or decreased: name the two most increased and two most decreased proteins of significant interest. What are the biological processes for these proteins?

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Biochemistry Question

Omics_Microbes:

 Introduction

Sepsis (also known as blood poisoning) is the immune system’s overreaction to an infection. Normally the human immune system fights infection but sometimes it attacks our body’s own organs and tissues. If not treated immediately, sepsis can result in organ failure and death. Yet with early diagnosis, it can be treated with antibiotics. Five people die each hour in the UK as a result of sepsis.

Study Design

Blood plasma samples were collected, processed and frozen from subjects visiting the X Hospital Accident and Emergency Unit and who were diagnosed with sepsis. Age and gender-matched control subjects were recruited from the same Accident and Emergency Unit and blood plasma was also collected, processed and stored; these control subjects were tested for but were not diagnosed with sepsis. The sepsis and control samples have been analysed applying a quantitative proteomics method; a specific protein has received a more in-depth mass spectrometry analysis; and the sepsis and control samples have been analysed applying an untargeted metabolomics method to identify changes in metabolites.

Assessment

This assessment will allow you to investigate the data acquired. The assessment is broken down in to three separate sections.

The assessment should be submitted as a short scientific report defining (a) the computational methods applied; (b) the data analysis results generated and (c) a discussion/conclusion section. The maximum word count is 800 words.

 

The data collected is presented in the file named ‘Proteomics data.xls’.

  1. Construct a volcano plot to visualise the changes in fold change and p values from health to sepsis. (By excel)
  2. If you assume p<0.05 is significant and prioritise two-fold increased or decreased: name the two most increased and two most decreased proteins of significant interest.
  3. What are the biological processes for these proteins?

 

Serum samples were extracted and analysed for 15 subjects diagnosed with sepsis and 15 subjects not diagnosed with sepsis. Plasma was extracted in 50/50 methanol/water (v/v). Each sample was analysed applying a ultra-performance liquid chromatography-mass spectrometry (LC-MS) method followed by raw data processing. Quality Control (QC) samples were also analysed as part of the study.

The processed LC-MS data is presented in the file named ‘Omics_Microbes_Metabolomics_dataset_Case_Study.csv’.

  1. Assess the quality of the data provided applying multivariate analysis methods. Define whether the data is of an adequate quality to perform statistical analysis.
  2. Applying univariate statistical analysis methods, identify the metabolites which show a statistical significance between subjects with sepsis and subjects without sepsis. Report the metabolite and statistical critical p-value for all metabolites with a critical p-value < 0.05 (FDR-corrected).
  3. Identify the metabolite pathway or pathways that are associated with the statistically significant metabolites described in part b.

 

Suppose you were creating a program to support pregnant women and their families in delivering healthy babies. Identify factors that you might address at each level of Bronfenbrenner’s model (Chapter 1). Discuss your program and how it may help promote healthy infants.

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Psychology of Infancy and Childhood

1. Most developmental scientists agree that nature and nurture interact to influence development. explain how sickle cell anemia being a genetic disorder illustrates the interaction of genes and context.

2. Mention two of the most common Infant Mental Health related disorders- explain them.

3. Suppose you were creating a program to support pregnant women and their families in delivering healthy babies. Identify factors that you might address at each level of Bronfenbrenner’s model (Chapter 1). Discuss your program and how it may help promote healthy infants.

4. Theo pushes into a crawling position onto all fours and rocks back and forth. Theo will soon crawl, about 2 months later than the other babies in the childcare center. Much smaller than his peers, Theo is new to the childcare center, recently adopted from an overcrowded orphanage in a developing country. At first tiny Theo didn’t eat much. The adults around him worried about his poor eating habits and lack of growth. Soon, however, Theo became comfortable in his new, affectionate and nurturing home and he began to grow quickly.

  •  How do motor skills unfold during infancy? Should Theo’s parents worry about his progress? Why or why not?
  • Theo’s mother worries about how his early experiences of deprivation might influence Theo’s brain development. Discuss processes of brain development and the role of experience in development.
  • What can she do to help Theo?

Write an essay explaining how nursing has evolved over time.

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Contemporary nursing

Write an essay explaining how nursing has evolved over time.

In chapter 7 we are told that intercultural relationships go through three phases. Explain what these phases are and provide an example of a relationship going through these phases (in a minimum of 150 words).

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Communications Question

ANSWER THE WRITTEN QUESTIONS FULL and correct in your own words

1) In chapter 7 we are told that intercultural relationships go through three phases. Explain what these phases are and provide an example of a relationship going through these phases (in a minimum of 150 words).

2) Explain what a use value and an exchange value are. In addition choose one product (any) and explain its importance in terms of their use and exchange values (in a minimum of 100 words)

3) One of the things that characterized or started the industrial revolution was that the connection between producers and the means of production was removed. What does this mean and please provide an example.

4) Explain what an independent and interdependent orientation. Also explain which orientation you believe yourself to be and why (in a minimum of 100 words)

5) Define 5 of the following 10 terms

  • Culture jamming
  • negotiated reading
  • in-group
  • capitalism
  • micro-frame analysis
  • media
  • heteronormativity
  • neoliberal policy
  • encoding
  • network media

Identify the 2 crimes you researched. Describe the sentencing guidelines in TN state for each crime. Compare the sentencing guidelines for adult offenders and juvenile offenders for each crime.

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Sentencing

Sentencing guidelines are determined through best practice research and public policies. Criminal justice practitioners must be well-informed regarding public policies and trends that impact sentencing in order to uphold best practices. In this summative assessment, you look at TN state’s sentencing guidelines for 2 crimes, as well as the public policies that influenced them.

Research examples of sentencing guidelines for 2 specific crimes in TN state and the public policies that influenced these guidelines.

Write a 1,750-word analysis of sentencing guidelines and the related public policies. Address the following in your paper:

  • Identify the 2 crimes you researched.
  • Describe the sentencing guidelines in TN state for each crime.
  • Compare the sentencing guidelines for adult offenders and juvenile offenders for each crime.
  • Compare the sentencing guidelines of TN state to the guidelines of TX state and the guidelines at the federal level for each crime.
  • Describe public policies and trends that influenced the sentencing guidelines in TN state for the crimes you researched.
  • Explain the impetus for the public policies and data that supported the public policies.
  • Propose a change to the sentencing policies in your state for the crimes researched and substantiate your proposal with support from research on the benefits of this change.

What types of art are featured here? What kinds of art are not represented here? How does the organization feel to you? Did you feel comfortable in this space—why or why not?

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Art Question

Hunt: During Visit

  • Find the works of art you selected within the museum. Set a timer for one minute and observe: What do you see? Write down three observations each (just notes, not full sentences).
  • Find a third work of art that interests you anywhere in the museum. Complete the same process.

Questions: After Your Visit

– The notes from each of the paintings with the painting name included.

– Choose one work and answer: How does this relate to what you’ve learned about the Italian Renaissance?

– About the museum generally: What types of art are featured here? What kinds of art are not represented here? How does the organization feel to you? Did you feel comfortable in this space—why or why not?

The Guerrilla Girls created provocative posters using appropriated images and confrontational text. Create your own provocative poster.

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Guerilla Girls

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Watch this video introduction to the Guerrilla girls.

Do women have to be naked to get into the Met. Museum? was an early poster created by the Guerrilla Girls in order to bring attention to racial and gender inequalities in the art world. Go to the Girls’ website Guerrilla Girls (Links to an external site.) to see more examples of posters and other mass media “ads” they made.

  • View at least ten different poster designs.
  • Think about: Which are the most effective? Which are the least effective? Why? Which is the most artistic? The least artistic?
  • Then: Guerrilla Art. The Guerrilla Girls created provocative posters using appropriated images and confrontational text. Create your own provocative poster. Appropriate an image from the textbook or online or draw one. You can put a gorilla mask on the figure if you like or reference the Guerilla Girls in another way. Incorporate research or factual text to address an issue of race, class, or gender.

Define Strategic Thinking. List the three ways in which companies formulate strategy. Explain any one rule of strategic thinking.

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Discussion

Q1- Read the article at the following link.

https://www.mckinsey.com/business-functions/strate…

(Question):

  1. Define Strategic Thinking.
  2. List the three ways in which companies formulate strategy.
  3. Explain any one rule of strategic thinking.

 

Q2- Watch the video at the following link.

(Question):

  1. List the five forces of “Porter’s five forces” industry framework.
  2. Illustrate Porter’s five forces industry framework through a local example.

 

Q3- Watch the short video at the following link

(Question):

  1. Describe Business Model.
  2. List the nine building blocks of the Business Canvas Model.
  3. Explain any one building block of the Business Canvas Model along with an appropriate example.

Select an article or podcast that seems interesting and relevant. Prepare a 1- to 2-page, double-spaced summary of the article/podcast that you chose.

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Insights on New Product Development

Product Development and Management AssociationLinks to an external site. Click “KHUB” in the upper right corner of the home page menu. Select an article or podcast that seems interesting and relevant. Prepare a 1- to 2-page, double-spaced summary of the article/podcast that you chose.

Demonstrate your ability to utilize course concepts and incorporate case studies in producing knowledge through cultural criticism in writing.

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Closing Lecture Remarks

Understanding capitalism’s influence over our everyday lives and not just the historical past is a lifelong endeavor most of us may be discouraged to undertake. Even so, this does not limit the role and impact of capitalism over our lives, decision-making, and prospects about what comes next for us as individuals, households, nation, and the world.

So much of our own circumstances are engrained in class differences produced by capitalism and it’s initial primitive accumulation through colonization and imperialism. In fact, who is eligible for citizenship and the path for each is different depending on your economic class under capitalism. For example, a Mexican millionaire can automatically qualify for U.S. citizenship when they make a million-dollar investment in the U.S. Meanwhile, a Mexican citizen may work all their adult lives in the U.S. paying taxes and contributing to their local community but be denied citizenship for crossing the border without proper documents.

Takaki casts Mexicans in A Different Mirror as immigrants, but that is not entirely true. Immigrants come from Asia, Europe, and Middle East. The indigenous populations of North America can hardly be described as immigrants. The notion of nations, however, turns many indigenous tribes and people who are natives into “immigrants” equatable to Europeans who came on ships. This example of who is assigned as an immigrant is another example of hegemony and its tremendous influence in the way we interpret knowledge and language to reproduce social relations that maintain elite rule and dominance.

Now is your opportunity to demonstrate your ability to utilize course concepts and incorporate case studies in producing knowledge through cultural criticism in writing.